Chromosome conformation capture (3C) analysis
October 25, 2007
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Chromosome conformation capture (3C) analysis
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To generate control templates for the positive controls, BAC clones were used for all the loci of interest. For the TH2 locus we used the BAC clone B182 …
www.nature.com/nature/journal/v435/n7042/extref/nature03574-s6.doc -
Random Shear BAC Library Construction
October 25, 2007
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Random Shear BAC Library Construction
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The quality of genomic BAC libraries depends greatly on the cloning methods and vectors …. BAC Cloning System simplifies BAC preparation and sequencing …
www.lucigen.com/catalog/images/pdfs/newsletters/Random_ShearBAC.pdf -
How the CopyControl™ BAC Cloning Kits Work
October 25, 2007
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How the CopyControl™ BAC Cloning Kits Work
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The CopyControl BAC Cloning Kits–. based on technology developed in the. laboratory of Dr. Waclaw Szybalski. 1. at the University of Wisconsin-Madison …
www.epibio.com/pdfforum/9_3ccbacclone.pdf
Protocol for CopyControl’ BAC Cloning Kit
October 25, 2007
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Protocol for CopyControl’ BAC Cloning Kit
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CopyControl™ Products including, but not limited to, the CopyControl BAC Cloning Kit are covered by U.S. Patent No. 5874259 licensed to …
www.epibio.com/pdftechlit/177pl055.pdf
Dynamic Building of a BAC Clone Tiling Path
October 25, 2007
Dynamic Building of a BAC Clone Tiling Path for the Rat Genome …
the sequence of each BAC clone is generated by assembling the … genome is obtained by merging overlapping BAC clone se-. quences. …
www.genome.org/cgi/reprint/14/4/679.pdf
Direct Cloning and Sequencing of Bacterial Artificial Chromosome
October 25, 2007
Direct Cloning and Sequencing of Bacterial Artificial Chromosome …
of BAC clone inserts is an essential step in chromosome walking and map- …. sites, one from the BAC cloning site and the other from the pBlueScriptII …
www.springerlink.com/index/G0U66PK677681N2K.pdf
BAC-CLONING AND MUTAGENESIS
October 25, 2007
BAC-CLONING AND MUTAGENESIS OF HERPESVIRUS GENOMES
Cloning as an infectious plasmid and mutagenesis in E.coli has become the method of choice for genetic analysis of viruses with small genomes. Until recently, this approch was not accessible for herpesviruses since their large genomes (150 to 230 kbp) could not be cloned in one piece in E.coli….
Read Full Article Here
http://www.lmb.uni-muenchen.de/groups/messerle/bac.htm
BAC Clone Ordering
October 25, 2007
BAC Clone Ordering
Only genome sequences determined by systematic bacterial artificial chromosome (BAC) clone sequencing will have clones available. Whole Genome Shotgun (WGS) clones are generally not available for ordering, but if the genome sequences are the result of a composite assembly, BAC clones may be available in certain regions. The human genome sequence however has been solely determined by systematic BAC clone sequencing…
Read full article here
http://www.ensembl.org/info/data/docs/clone_ordering.html
How to identify an address
October 25, 2007
How to identify an address:
The membrane is divided into 6 fields (diagram provided). Each field contains 384 squares. The 384 squares represent the row and column identification of the BAC. Within each square there are 16 positions where 8 clones are spotted in duplicate (diagram). The pattern of the spotted clones will generate the plate address of the BAC. To identify your clone, please follow the directions below.
The most complicated part about identifying a clone address is that consecutive plates are not spotted into each field. The 384 well plates are spotted onto the membrane with plates 1-6 spotted into fields 1-6 respectively (duplication pattern 1, see diagram). Since there is a total of 6 fields on the membrane, the cycle will continue with the next six consecutive plates (plates 7 through 12) again being spotted into fields 1 through 6 respectively, but in a different duplication pattern (duplication p…..
Full Protocols Here
http://www.genome.clemson.edu/groups/bac/protocols/addressnew.html
Megabase-size DNA isolation from plants
October 25, 2007
Megabase-size DNA isolation from plants
To construct large insert DNA libraries in BAC and YAC vectors, methods must be developed to isolate very high molecular weight DNA - megabase-size DNA - from plants. To isolate such DNA, protoplasts or nuclei must first be embedded in agarose plugs or microbeads. The agarose acts as a solid yet porous matrix which allows for the diffusion of various reagents for DNA purification and subsequent manipulations while preventing the DNA from being sheared (Schwartz and Cantor, 1984). Microbeads are preferred over plugs because the use of beads increases the surface area surrounding the tissue sample by approximately 1000 fold thereby allowing for more efficient and rapid diffusion of chemicals and enzymes into and out of the agarose beads (Cook, 1984, Overhauser and Radic, 1987, Wing……….
Read Full Protocol Here
http://www.genome.clemson.edu/groups/bac/protocols/megabasedna.html